mGRASP enables mapping mammalian synaptic connectivity with light microscopy

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Improved synapse detection for mGRASP-assisted brain connectivity mapping

MOTIVATION A new technique, mammalian green fluorescence protein (GFP) reconstitution across synaptic partners (mGRASP), enables mapping mammalian synaptic connectivity with light microscopy. To characterize the locations and distribution of synapses in complex neuronal networks visualized by mGRASP, it is essential to detect mGRASP fluorescence signals with high accuracy. RESULTS We develope...

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ژورنال

عنوان ژورنال: Nature Methods

سال: 2011

ISSN: 1548-7091,1548-7105

DOI: 10.1038/nmeth.1784